![]() ![]() With UDR, IM transport rate decreased and AFV increased. UD decreased IM transport rate and AFV in conjunction with enhanced expression of vesicular endocytosis regulators but reduced expression of intracellular trafficking mediators. These can be categorized into mediators of vesicular uptake and endocytosis, intracellular trafficking, pathway activation and signaling, and energy metabolism. The analysis uncovered nine transport-associated pathways and four groups of differentially expressed transcripts and proteins. ![]() Amnion, AF, and fetal urine were subjected to transcriptomics (RNA-Seq) and proteomics studies followed by Ingenuity Pathway Analysis. Four groups of fetal sheep with experimentally induced alterations in IM transport rate were studied: control, urine drainage (UD), urine drainage with fluid replacement (UDR), and intra-amniotic fluid infusion (IA). Our objective was to utilize a multiomics approach to determine the IM transport pathways and identify the regulators. This transport is regulated by fetal urine-derived stimulators and AF inhibitors. Amniotic fluid volume (AFV) is determined by the rate of intramembranous (IM) transport of amniotic fluid (AF) across the amnion. ![]()
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